Shanghai Jinma summary ELISA test kits frequently asked questions and answers - Database & Sql Blog Articles

As the first half of 2016 came to a close, Shanghai Jinma took time to reflect on its progress. This success is driven by customer support and the hard work of our team. We are grateful for your trust and collaboration, which have helped us grow and achieve great results. During this period, we received many inquiries, and our technical team was always ready to assist with experiments and answer questions.

In order to better serve our customers, we’ve compiled and analyzed the most frequently asked questions about the ELISA test kits (http://elisa.app17.com/shyksw-Products/T110729.html). Below are some common questions and answers that may help you understand the process better:

(1) Q: Mouse acetylcholine Elisa Detection – What is the role of the blank OD value in data processing?

A: You can either subtract the blank value from the standard and sample OD values or keep them as they are, as long as consistency is maintained across all measurements.

(2) Q: Why do we sometimes get a negative ACH content value?

A: This usually indicates that the sample concentration is very low. When it's near the upper limit of the standard curve, the linear equation might result in a negative value due to calculation limitations.

(3) Q: I didn’t add one of the two blank wells. One had only A liquid added later, and B liquid along with stop solution. Which one should be used?

A: It’s more accurate to add A liquid, B liquid, and stop solution in all blanks. This helps eliminate potential interference and ensures better accuracy.

(4) Q: Is there a linear relationship between standard concentration and absorbance? Why are the detected absorbance values so low (around 0.08)?

A: Yes, the relationship is typically linear. Low values can be caused by sample preparation, dilution, or operational errors. As long as they fall within the acceptable range, they are still valid.

(5) Q: The standard curve shows a linear regression, but the sample absorbance is close to the background. Why is there no 5-HT expression in normal rat plasma?

A: This could be due to sample handling, dilution, or machine operation. In some cases, using a fluorescence-based method might provide more accurate results.

(6) Q: What is the detection method used in the kit?

A: It uses a double-antibody sandwich method, which is currently the standard approach.

(7) Q: How much serum should be added per well?

A: Typically, 10–50 microliters per well is sufficient.

(8) Q: How much unprocessed sample should be sent?

A: For each well, 100 microliters is recommended.

(9) Q: How should samples be stored?

A: If used the same day, store at 4°C. For short-term storage (up to a month), use -20°C. For long-term storage, -70°C is ideal. Avoid repeated freeze-thaw cycles.

(10) Q: What is an EP tube?

A: It is a centrifuge tube commonly used in laboratory settings.

(11) Q: What kind of tube should be used for collecting serum?

A: A regular centrifuge tube is suitable for this purpose.

(12) Q: How many samples can a 96T and 48T kit detect?

A: A 96T kit can detect up to 85 samples, with 10 standards and 1 blank control. A 48T kit can detect around 37 samples, with similar controls.

(13) Q: What is the benefit of using a standard test instead of single-hole testing?

A: The standard test, also known as double-hole detection, improves repeatability and reliability of results.

(14) Q: How should unused ELISA plates be stored?

A: If not needed immediately, remove the plate from the foil bag and store it at 2–8°C in a dry place. They can be reused if properly handled.

(15) Q: Is an enzyme-labeled coating plate the same as an ELISA plate?

A: Yes, they are essentially the same product.

(16) Q: What is the typical sampling amount when extracting Arabidopsis for jasmonic acid and salicylic acid detection?

A: Around 1–3 grams is standard for such experiments.

(17) Q: My sample concentration exceeds the minimum detection limit. Should I dilute the standard?

A: The special diluent provided in the kit is intended for both standards and samples. If the sample is too concentrated, it should be diluted accordingly.

(18) Q: Why did my R² value drop from 0.98 to 0.93?

A: If a point deviates significantly, it can be excluded, and the remaining five points can be used for the curve.

(19) Q: What is a blank control?

A: It refers to a well where no sample or reagent is added, serving as a baseline for background subtraction.

(20) Q: Is the sample diluent pre-prepared?

A: Yes, it comes pre-mixed and ready for use.

(21) Q: Can the sample diluent be used directly to dilute serum?

A: Yes, you can add 40 µL directly to the sample.

(22) Q: Do I need to perform the experiment in a biosafety cabinet?

A: Not strictly necessary. It depends on your lab setup and safety protocols.

(23) Q: Is the washing solution for human copeptin ELISA concentrated or ready-to-use?

A: It is concentrated and requires dilution before use.

(24) Q: Can ELISA measure IgG antibody titer in human platelets?

A: If ELISA is not suitable, consider using a fluorescence-based method instead.

(25) Q: Does an uneven ELISA plate affect the results?

A: No, it does not impact the final outcome.

(26) Q: Can ELISA measure TNF-α inhibition in different groups?

A: Yes, cells can be tested, and results can be calculated based on group comparisons. Further literature review may be helpful for detailed interpretation.

(27) Q: What precautions should be taken when using testosterone or estriol ELISA kits?

A: Avoid adding sodium azide to horseradish peroxidase-labeled samples.

(28) Q: How should tobacco leaf samples be prepared for salicylic acid detection?

A: Grind 500 mg of leaves, mix with 5 mL PBS (pH 7.4), centrifuge, and collect the supernatant. No methanol is required unless specified.

(29) Q: Why wasn't a standard curve made?

A: This likely indicates an error in the procedure. Make sure to shake the standard before running the test.

(30) Q: Should I make a standard curve for each box of the same kit?

A: Yes, even if you use multiple boxes, it’s best to create a standard curve for each batch to ensure consistency.

Shanghai Jinma Experimental Equipment Co., Ltd. is dedicated to advancing enzyme-linked immunosorbent technology, combining expertise from both domestic and international professionals. By utilizing advanced technology and equipment, we aim to deliver high-quality products at competitive prices, supporting researchers and contributing to national scientific development. With a strong team and numerous bio-research partners, we focus on the production and R&D of ELISA kits to help your research succeed. Let’s continue to innovate and drive science forward together!