Difference between centrifuge angle rotor and horizontal rotor - Database & Sql Blog Articles

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Centrifugal rotors are most commonly divided into two main types: angle rotors and horizontal rotors.

1. What is an angle rotor?

An angle rotor, also known as a fixed-angle rotor, is designed with a specific angle θ between the centrifuge tube and the rotating shaft during manufacturing. This angle remains constant throughout the process and typically ranges from 14° to 45°. During centrifugation, the tubes remain in a fixed position, and the sample moves toward the bottom of the tube. The smaller the angle between the tube and the axis, the more efficient the sedimentation, as the particles travel a shorter distance before settling.

2. What is a horizontal rotor?

A horizontal rotor, sometimes referred to as an open rotor, operates differently. In this type, the centrifuge tubes are placed in individual hanging cups that are attached to the rotor. When the rotor is stationary, the cups are positioned vertically. However, when the rotor spins, centrifugal force causes the cups to swing outward and align horizontally. It's essential to ensure the cups are symmetrically placed on the rotor for balanced operation. This design allows for easier loading and unloading of samples, making it ideal for certain applications such as density gradient centrifugation.

Understanding the differences between these two types of rotors helps in selecting the right one based on the nature of the sample and the desired outcome of the centrifugation process.