In order to ensure the stability and smooth execution of ELISA kit experiments, most researchers and distributors must first focus on product selection. It is crucial to choose high-quality products that guarantee reliability rather than simply opting for cheaper alternatives that may compromise results. When using ELISA kits, it's essential to maintain strict standards and follow proper procedures. Here are some key requirements to consider when using ELISA kits:
â— The laboratory technician should have the necessary skills and hands-on experience in performing lab operations. They should be proficient in using lab instruments and capable of identifying and resolving issues that may arise during the experiment.
â— Use a calibrated micropipette to minimize human error. Accuracy in pipetting is especially critical for quantitative detection, as even small deviations can affect the final results.
◠Always read and strictly follow the manufacturer’s instructions. Do not mix reagents from different batches, as this can lead to inconsistent outcomes. If improvements are needed, they should be tested and validated before implementation.
â— Pay attention to the shelf life of the ELISA kit and its reagents. Expired reagents should never be used, as they can significantly impact the accuracy and reliability of the test.
â— Evaluate the performance of the reagents. Stability is key to ensuring accurate results. Before activating the reagents, perform control tests with positive and negative samples to verify their functionality.
Additionally, our technical team has emphasized that once the ELISA reagents are opened, they should be used within one week. Any remaining reagents should be carefully checked for signs of degradation before the next use. Contaminated color reagents, for example, can cause false readings and compromise the entire experiment.
It’s always better to be cautious and attentive when working with ELISA kits. Proper handling and adherence to guidelines can greatly enhance the quality of your results. If you have any questions or need further assistance, feel free to reach out to us at any time.
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